Table 3 Species specific sensitivity, specificity, positive predictive value and negative predictive value of RDTs compared with HVUSqPCR parasite detection
qPCR pos, RDT pos | qPCR pos, RDT neg | qPCR neg, RDT neg | qPCR neg, RDT pos | Total | Sensitivity (95 % CI) | Specificity (95 % CI) | Positive predictive value (95 % CI) | Negative predictive value (95 % CI) | |
|---|---|---|---|---|---|---|---|---|---|
Pf | |||||||||
All sites | 96 | 120 | 4462 | 55 | 4733 | 44 % (38–51 %) | 99 % (98–99 %) | 64 % (55–71 %) | 97 % (97–98 %) |
Cam | 1 | 35 | 1411 | 0 | 1447 | 3 % (0–15 %) | 100 % (99–100 %) | 100 % (3–100 %) | 98 % (97–98 %) |
TMBA | 71 | 34 | 1241 | 37 | 1383 | 68 % (58–76 %) | 97 % (96–98 %) | 66 % (56–75 %) | 97 % (96–98 %) |
Viet | 24 | 51 | 1810 | 18 | 1903 | 32 % (22–44 %) | 99 % (98–100 %) | 57 % (41–72 %) | 97 % (96–98 %) |
Non Pf | |||||||||
All sites | 54 | 332 | 4332 | 15 | 4733 | 14 % (11–18 %) | 100 % (99–100 %) | 78 % (67–87 %) | 93 % (92–94 %) |
Cam | 0 | 52 | 1395 | 0 | 1447 | 0 % (0–1 %) | 100 % (93–100 %) | NA | 96 % (95–97 %) |
TMBA | 47 | 180 | 1153 | 3 | 1383 | 21 % (16–27 %) | 100 % (99–100 %) | 94 % (84–99 %) | 87 % (85–88 %) |
Viet | 7 | 100 | 1784 | 12 | 1903 | 7 % (3–13 %) | 100 % (99–100 %) | 37 % (16–62 %) | 95 % (94–96 %) |
P. spp. | |||||||||
All sites | 20 | 379 | 3740 | 46 | 4185 | 5 % (3–8 %) | 99 % (98–99 %) | 30 % (20–43 %) | 91 % (90–92 %) |
Cam | 0 | 145 | 1218 | 0 | 1363 | 0 % (0–0.3 %) | 100 % (97–100 %) | NA | 89 % (88–91 %) |
TMBA | 16 | 158 | 873 | 24 | 1071 | 9 % (5–15 %) | 97 % (96–98 %) | 40 % (25–57 %) | 85 % (82–87 %) |
Viet | 4 | 76 | 1649 | 22 | 1751 | 5 % (1–12 %) | 99 % (98–99 %) | 15 % (4–35 %) | 96 % (95–97 %) |