Fig. 4

Effect of amitozyn on the parasite developmental stage distribution. Plasmodium falciparum (3G8) culture was synchronized at the ring stage using 5 % d-sorbitol. iRBC were treated with 0–125 μg/mL amitozyn for 24, 48, 72, and 96 h and then stained by Giemsa’s method. The relative ratio of rings, trophozoites and schizonts was analysed by inverted light microscope as described in “Methods”