Fig. 5

Combined phylogenetic trees generated by maximum likelihood (ML) (left part) and neighbour joining (NJ) (right part) methods using the partial sequences of the asl gene from different Plasmodium species. Of the sequences in the dataset, the 15 obtained in the present study are in boldface type (cases imported in Singapore: 8 P. o. curtisi (dark green box), 3 P. o. wallikeri (light green box), with 1 P. vivax, 1 P. falciparum, 1 P. knowlesi, and 1 P. malariae), the other 15 Plasmodium sequences (GenBank accession number is provided between vertical bars) from Plasmodium species that infect humans and primates (P. falciparum and Plasmodium reichenowi, red box; P. malariae, yellow box; Plasmodium fragile, Plasmodium simiovale, Plasmodium inui, Plasmodium cynomolgi, P. knowlesi and P. vivax, blue box), apes, rodents (Plasmodium yoelii, Plasmodium berghei, Plasmodium vinckei, and Plasmodium chabaudi, purple box) and birds (Plasmodium gallinaceum, orange box). The homologous fragment from the Toxoplasma gondii asl gene was used at the out-group to root the trees. After alignment the sequences have been trimmed (≈800 bp) to adjust their size to the fragment with the most coverage