Table 2 Different clusters observed, with suggested genotype calls obtained from both TaqMan and ddPCR data followed by K-means clustering analysis
N° | Genotype cluster (gc) | Samples | Estimates genotypes |
|---|---|---|---|
1 | I | Okyereko | Gn: Homozygous susceptible individuals (Ace-1 nSS) with Ace-1 gene copy number greater than or equal to 1 |
2 | Boromo G3 | ||
3 | Kisumu 1 | ||
4 | Kisumu 8 | ||
5 | Sikensis 12 | ||
6 | Daloua 11 | ||
7 | Sikensis 1 | ||
8 | Kisumu 3 | ||
9 | II | Vallée du Kou | GnS: Heterozygous individuals (Ace-1 GnS) with more susceptible copies than resistant copies |
10 | Boromo 25 | ||
11 | Dano C7 | ||
12 | Boromo B2 | ||
13 | Dano 33 | ||
14 | Dano 34 | ||
15 | Divo 5 | ||
16 | Dano D6 | ||
17 | Cape-Coast 5 | ||
18 | Divo 1 | ||
19 | III | Divo 3 | (GS)n: Heterozygous individuals (Ace-1 (GS)n) with equal of susceptible and resistant allele and Ace-1 gene copy number greater than or equal to 1 |
20 | DD2 | ||
21 | Orodara D11 | ||
22 | DD1 | ||
23 | Divo 7 | ||
24 | Tiassalé 21 | ||
25 | IV | Orodara 8.22 | GSn: Heterozygous individuals (Ace-1 GSn) with more resistant allele than susceptible one |
26 | Orodara 8.10 | ||
27 | Orodara 6.4 | ||
28 | Orodara 8.13 | ||
29 | Baguida 99 | ||
30 | Toumodi 8 | ||
31 | Toumodi 2 | ||
32 | Koforidua 7 | ||
33 | Baguida 77 | ||
34 | V | Ashaman 5 | Sn: homozygous resistant individuals (Ace-1 Sn) with Ace-1 gene copy number greater than or equal to 1 |
35 | Koforidua 18 | ||
36 | Ashaman 4 | ||
37 | Koforidua 3 | ||
38 | Orodaea A9 | ||
39 | Acerkis | ||
40 | Baguida 6.5 | ||
41 | Ashaman 9 |