Detection of Plasmodium using filter paper and nested PCR for patients with malaria in Sanliurfa, in Turkey

Malaria Journal

Table 1 Schematic representation of the Plasmodium ssrRNA genes and nPCR protocol [13]

Species	PCR product	Primer	Sequence	Reaction
Plasmodium genus-specific	235 bp	rPLU3 rPLU4	TTTTTATAAGGATAACTACGGAAAAGCTGT TACCCGTCATAGCCATGTTAGGCCAATACC	Nested 2
Plasmodium genus-specific	1.6–1.7 kb	rPLU1 rPLU5	TCAAAGATTAAGCCATGCAAGTGA CCTGTTGTTGCCTTAAACTTC	Nested 1
Plasmodium species-specific
P. falciparum	206 bp	rFAL1 rFAL2	TTAAACTGGTTTGGGAAAACCAAATATATT ACACAATGAACTCAATCATGACTACCCGTC	Nested 2
P. malaria	145 bp	rMAL1 rMAL2	ATAACATAGTTGTACGTTAAGAATAACCGC AAAATTCCCATGCATAAAAAATTATACAAA	Nested 2
P. ovale	226 bp	rOVA1 rOVA2	ATCTCTTTTGCTATTTTTTAGTATTGGAGA ATCTAAGAATTTCACCTCTGACATCTG	Nested 2
P. vivax	121 bp	rVIV1 rVIV2	CGCTTCTAGCTTAATCCACATAACTGATAC ACTTCCAAGCCGAAGCAAAGAAAGTCCTTA	Nested 2

ISSN: 1475-2875