Table 1 Details of SMFA methods
Parameters | LMVR | TropIQ | |
|---|---|---|---|
All tests | mAbs | Human pAb | |
Gametocyte culture | |||
Flask | 25 or 75 cm2 flask | Tipper culture flask | |
Htca (%) | 5.0 | 5.0 | 5.0 |
Feeding | |||
Total volume (μL) | 260 | 270 | 1200 |
Liquid volume (μL) | 160 | 119 | 696 |
Liquid contents | 100 μL of human serum plus 60 μL of sample in 1xPBS | 119 μL of human serumb | 696 μL of human serumb |
Htcc (%) | 38.5 | 55.9 | 42.0 |
Stage V gametocyted (%) | 0.1–0.2 | 0.1–1.0 | NDe |
Starving mosquitoes (h) | 18–21 | 1 | 1 |
Time of feed (min) | 20 | 10 | 10 |
Elimination of unfed mosquitoes from analysis | Only mosquitoes with eggs at the time of dissection are analysed | One hour after feeding, fully fed mosquitoes are selected transported to a bigger cage | |
Dissection | |||
Days post feed (day) | 8 | 8 | 8 |
Number of mosquitoes | 20 | 20 | 30 |
Quality control | |||
Pre-feeding | >0.5 % of stage V gametocytaemia and >800/mL of exflagellating centres in the undiluted culture | Positive for male exflagellating gametocytes | |
Post-feeding | >4 oocysts/midgut in the assay controls | >70 % infected mosquitoes in the assay controls | |