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Table 1 LDR primers and microspheres used to detect G6PD polymorphisms

From: Prevalence and genetic variants of G6PD deficiency among two Malagasy populations living in Plasmodium vivax-endemic areas

Polymorphisma

LDR primersb

Microsc

A-

5′-tacaacatctcattaacatatacaAGGCGGGAACGGGCATAGCCCAC-3′

37

202G > A

5′-caaacaaacattcaaatatcaatcAGGCGGGAACGGGCATAGCCCAT-3′

22

rs1050825

common5′-Phos-GATGAAGGTGTTTTCGGGCAGAA-Biotin-3′

 

A

5′-cttaacatttaacttctataacacTGTGACCCCAGGTGGAGGGCATT-3′

30

3769A > G

5′-tacattcaacactcttaaatcaaaTGTGACCCCAGGTGGAGGGCATC-3′

26

rs1050829

common5′-Phos-CATGTGGCTGTTGAGGCGCTGGT-Biotin-3′

 

Mediterranean

5′-aatttcttctctttctttcacaatTCTGGTCCTCACGGAACAGGGAGG-3′

14

188C > T

5′-cacttaattcattctaaatctatcTCTGGTCCTCACGGAACAGGGAGA-3′

28

rs5030868

common5′-Phos-AGATGTGGTTGGACAGCCGGTCA-Biotin-3′

 

Silent

5′-cataatcaatttcaactttctactAAGACGTCCAGGATGAGGCGCTCG-3′

12

1311C < T

5′-acttatttcttcactactatatcaAAGACGTCCAGGATGAGGCGCTCA-3′

34

rs2230037

common5′-Phos-TAGGCGTCAGGGAGCTTCACGTT-Biotin-3′

 
  1. aEach polymorphic site includes information regarding legacy allele names, cDNA nucleotide change and RefSNP accession ID (rs)
  2. bLDR-FMA probes are based on positive-strand sequence. Polymorphic nucleotides are underlined and bold. Lower case letters represent 24 bp tags complementary to unique microsphere anti-tags
  3. cUnique microsphere sets designed by Luminex Corp