Fig. 1

Flowchart of malaria gametocyte viability screens and compound confirmation in gametocyte, asexual parasites and mammalian cell assays. a The primary screens of the Sytravon libraries were carried out in malaria P. falciparum gametocyte viability assays. A group of 128 hits from the malaria gametocyte screen were selected for confirmation in the same assays. Further, 32 hits were picked for inhibition of asexual parasites growth and tested for cytotoxicity in the mammalian HepG2 cell line. b Venn-diagram of confirmed hits with activities against P. falciparum gametocytes, P. falciparum asexual parasites, and the mammalian HepG2 cells. NCGC00104528 was clustered as active against both gametocytes and asexual parasites in Venn diagram (IC₅₀ of 8.91 µM in gametocyte viability assay and IC₅₀ of 13.9 µM in asexual parasite growth assays)