Fig. 2
Pfs25 specific Ig production and T-cell activation following Ad5-pfs25 primary vaccination. Mice (n = 3 per group) were immunized with Ad5 expressing Pfs25 (1010 particles) or recombinant yPfs25-alum (2.5 µg/mouse) on day 0, and serum and splenocyte samples were collected on day 10. a yPfs25-specific ELISA, yPfs25-specific ELISA were performed and data depict pooled serum from 3 animals per group tested in triplicate in 3 independent ELISAs. The average ELISA unit and standard deviation are plotted. b Splenocyte IFNγ secretion. Splenocytes (n = 3, tested individually) were incubated without or with yPfs25 at 10 μg/ml or Ad5 at 10,000 particles per cell for 24 h as indicated on the x-axis. IFNγ secretion was detected by ELISA and the average and standard deviation are plotted. Statistical significance was determined by Student’s T test; ***p < 0.001