Fig. 5

Experimental cerebral malaria (ECM) is suppressed by deficiency of parasite MQO but not FH. Female C57BL/6 mice were infected with 1 × 10⁴ infected erythrocytes of control, Δfh and Δmqo parasites. a Course of parasitaemia. Asterisks indicate a statistically significant difference (*, control vs. Δfh-infected mice; **, control vs. Δmqo-infected mice). b Survival rate. Note that neurologic signs of ECM were observed in mice infected with control and Δfh parasites on day 7 post-inoculation. c Brains of mice injected with Evans blue (top panels). Brains were obtained from uninfected and control- and Δfh-infected mice on day 7 post-infection, and from Δmqo-infected mice on days 7 and 14 post-infection. Scale bar 10 mm. Quantitative analysis of Evans blue extravasation in the brain (bottom). Results are expressed as mean ± SD of three mice. Experiments were performed in duplicate, and representative data are shown. Asterisks indicate statistically significant differences (**, control vs. Δmqo-infected mice)