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Fig. 3 | Malaria Journal

Fig. 3

From: Assessment of the quality and quantity of naturally induced antibody responses to EBA175RIII–V in Ghanaian children living in two communities with varying malaria transmission patterns

Fig. 3

Characterization of IgG responses against EBA175RIII–VLl. Antibody concentrations (ng/ml) of plasma obtained from the enrolled children from Obom and Abura (a) diluted 1:200 was determined using indirect ELISA and a EBA175RIII–VLl as the antigen coated onto the ELISA plate and goat anti-human IgG used as the secondary antibody. The relative avidities of these same plasma samples were determined using a modified indirect ELISA assay where an incubation of the bound plasma samples obtained from children Obom and Abura (b) were treated with sodium thiocyanide is incorporated into the protocol. Plasma samples were obtained from whole blood collected from the children during the months of July 2015, October 2015 and February 2016. Data in the graphs are represented as the median with the interquartile range

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Malaria Journal

ISSN: 1475-2875

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