Table 1 Primer and probe sequences used in this study

PerFal

5′-CTTTTGAGAGGTTTTGTTACTTTGAGTAA-3′

P. falciparum 18S rRNA gene

[31]

5′-TATTCCATGCTGTAGTATTCAAACACA-3′

5-FAM-TGTTCATAACAGACGGGTAGTCATGATTGAGTTCA-BHQ1′

RPS7

5′-TGGAAATGAACTCGGATCTGAAG-3′

An. stephensi rRNA protein S7 gene (RPS7)

[41]

5′-CCTTCTTGTTGTTGAACTCGACCT-3′

This study

5′-HEX-CAGCTGCGTGATCTGTACATCACCCGCGC BHQ1′

This study

EHV

5′-GATGACACTAGCGACTTCGA-3′

Equine Herpesvirus

[42]

5′-CAGGGCAGAAACCATAGACA-3′

5′-QUASAR670 -TTTCGCGTGCCTCCTCCAG-BHQ1′