Fig. 4

NAPPA functional assessment of Plasmodium vivax proteins. a The Pv12 protein was used as query for evaluating its interaction with 29 P. vivax protein fragments displayed on the array and one P. falciparum protein (PfMSP1_83kDa). Query DNA encoding an N-terminal Halo tag was added to reticulocyte lysate for co-expression with bait proteins. The interaction signal was determined with anti-Halo antibodies. Normalized values are shown for 30 clones evaluated on the array. A median value > 1 was considered positive interaction. b Median value ± standard deviation of proteins interacting with Pv12