Table 1 Sequences of primers and probes used in the LNA kdr assay with 5′ and 3′ modifications indicated
Name | 5′ Fluorescence modification | Sequence | 3′ Quencher modification |
|---|---|---|---|
VGSC-F | CGTGTGCTATGCGGAGAATG | ||
VGSC-R | CGATCTTGGTCCATGTTAATTTGC | ||
Kdr-Leu | HEX | A+CGA+C+T+AAAT+TTC+C | IBFQ |
Kdr-Phe | 6-FAM | A+CGA+C+A+AAAT+TTC+C | IBFQ |
Kdr-Ser | Cy5 | +CGA+C+T+GAAT+TTC+C | IBRQ |