Table 1 Genotypes and their frequencies of propeller domain in the k13 gene from P. falciparum isolates of Yunnan Province and Myanmar

Sequences of k13 gene

–

384

194

190

Wild genotype

Hap_1

–

–

202

126

76

Synonymous mutations

–

 G449G

Hap_17

c.1347 T > G

GGT > GGG

1 (0.3)

0

1 (0.5)

 T451T

Hap_13

c.1353 T > C

TTT > TTC

1 (0.3)

1 (0.5)

0

 G674G

Hap_22

c.2022 G > A

GGG > GGA

1 (0.3)

0

1 (0.5)

Single mutants genotype

–

 F446I*

Hap_2

c.1338 T > A

TTT > ATT

130 (33.6)

49 (25.3)

81 (42.6)

 G450V*

Hap_15

c.1349 G > T

GGA > GTA

1 (0.3)

0

1 (0.5)

 N458Y*

Hap_7

c.1372 A > T

AAT > TAT

3 (0.8)

2 (1.0)

1 (0.5)

 S459L*

Hap_9

c.1376 C > T

TCG > TTG

2 (0.5)

2 (1.0)

0

 C469Y*

Hap_10

c.1407 G > A

TGC > TAC

4 (1.0)

1 (0.5)

3 (1.6)

 A481V*

Hap_20

c.1442 C > T

GCT > GTT

2 (0.5)

0

2 (1.0)

 F483S*

Hap_18

c.1448 T > C

TTT > TCT

1 (0.3)

0

1 (0.5)

 L492S*

Hap_19

c.1475 T > C

TTA > TCA

1 (0.3)

0

1 (0.5)

 G533A*

Hap_12

c.1598 G > C

GGT > GCT

2 (0.5)

1 (0.5)

1 (0.5)

 P553L*

Hap_16

c.1658 C > T

CCG > CTG

3 (0.8)

0

3 (1.6)

 E556D*

Hap_5

c.1668 A > T

GAA > GAT

3 (0.8)

1 (0.5)

2 (1.0)

 P574L*

Hap_8

c.1721 C > T

CCT > CTT

7 (1.8)

2 (1.1)

5 (2.6)

 A578S*

Hap_11

c.1732 G > T

GCT > TCT

1 (0.3)

1 (0.5)

0

 C580Y*

Hap_14

c.1739 G > A

TGT > TAT

4 (1.0)

0

4 (2.1)

 V581I*

Hap_3

c.1741 G > A

GTT > ATT

1 (0.3)

1 (0.5)

0

 E668D*

Hap_4

c.2004 G > C

GAG > GAC

1 (0.3)

1 (0.5)

0

 A675V*

Hap_23

c.2024 C > T

GCT > GTT

1 (0.3)

0

1 (0.5)

 A676D*

Hap_6

c.2027 C > A

GCC > GAC

11 (2.9)

6 (3.2)

5 (2.6)

Double mutants genotype

–

 Y519K*

Hap_21

c.1855 T > A, c.1857 T > G

TAT > AAG

1 (0.3)

0

1 (0.5)

 G449G/A676D*

Hap_17

c.1337T > G, c.2027 C > A

GGT > GGG,GCC > GAC

Number was the same above synonymous mutations

Yes

Haplotype

23

12

18

Ka/Ks

12.2

16.3

10.9

He

0.6124

0.0481

0.044

H

0.0283

0.5182

0.651

Fst (P value)

–

0.0410^A (0.0020^B)