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Fig. 2 | Malaria Journal

Fig. 2

From: Validation of Plasmodium falciparum dUTPase as the target of 5′-tritylated deoxyuridine analogues with anti-malarial activity

Fig. 2

Analysis of mutants overexpressing PfdUTPase and HsdUTPase. a Immunofluorescence analysis of 3D7 wild-type cells (upper panels) and mutants overexpressing PfdUTPase (middle panels) and HsdUTPase (lower panels). A monoclonal antibody for PfdUTPase and a polyclonal antibody for HsdUTPase were used. Images were obtained using a confocal Leica TCS SP5 microscope and show a single optical plane. b Western blot analysis indicating the correct overexpression of PfdUTPase (left panel) and HsdUTPase (right panel) in parasites transfected with constructs where the coding sequence was cloned into pHH2. Western blotting was performed with polyclonal antibodies raised against PfdUTPase and HsdUTPase respectively. Hsp70 was used as loading control

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Malaria Journal

ISSN: 1475-2875

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