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Fig. 3 | Malaria Journal

Fig. 3

From: Validation of Plasmodium falciparum dUTPase as the target of 5′-tritylated deoxyuridine analogues with anti-malarial activity

Fig. 3

Effect of dUTPase inhibitors on cell growth and nucleotide pools. a Resistance induced in Plasmodium cultures by overexpression of different dUTPases indicated by the fold change observed in the value of IC50 obtained for transfected Plasmodium falciparum lines overexpressing PfdUTPase (PfdUTPase OE) and human dUTPase (HsdUTPase OE) with regard to the parental line 3D7. Chloroquine was used as reference compound. b Measurements of the dTTP (left panel) and dUTP pools (right panel) after incubation for 20 h with compounds 3 and 4 at different concentrations (IC50 and threefold the IC50). Determinations of IC50 values and nucleotide levels were performed in triplicate and the experiments were performed twice. Average values of both experiments are indicated and error bars represent the standard error. The asterisks show significant differences calculated by the Student’s t-test. **p < 0.01

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Malaria Journal

ISSN: 1475-2875

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