Fig. 2
From: Human plasma plasminogen internalization route in Plasmodium falciparum-infected erythrocytes
Transmission electron microscopy micrographs of Plasmodium falciparum-infected erythrocytes with immunogold-labelled plasminogen. Uninfected and infected erythrocytes were analysed by immunoelectron microscopy (immunogold and silver enhancement method) with an antibody against angiostatin. a The immunogold particles (black dots) are associated with uninfected erythrocyte plasma membrane; b iRBCs with parasites at the trophozoite stage; c, d magnified images of the areas indicated in (b). Images show the presence of human plasminogen in the parasite cytosol (not significant inside organelles) and its presence in the three membranes (RBC, PVM and PM). Food vacuoles (FVs) containing residual hemozoin (HZ) and endocytic vesicles (EVs) of RBC haemoglobin are not labelled. The analyses also show the human protein in and attached to the tubovesicular network (TVN) and e Maurer’s clefts (MC)