Primers | Amplicon sizes (bp) | PCR cycling programs |
---|---|---|
Outer forward FN1/15_- AGGTTGAAAAAGAGTTGAAC Outer reverse REV/C15_-ATGACACCACAAACATAAAT | 578 | 34 cycles of 94 °C for 30 s; 55 °C for 30 s; and 65 °C for 1 min; then 65 °C for 5 min and 4 °C forever 34 cycles of 94 °C for 30 s; 55 °C for 1 min; and 65 °C for 1.5 min; then 65 °C for 5 min and 4 °C forever |
Outer forward MDRFR2F15_-GTGTATTTGCTGTAAGAGCT Outer reverse MDRFR2R15_-GACATATTAAATAACATGGGTTC | 958 | |
Nested forward MDR2/15_-ACAAAAAGAGTACCGCTGAAT Nested reverse NEWREV15_-AAACGCAAGTAATACATAAAGTC | 534 | 29 cycles of 94 °C 30 s; 60 °C for 30 s; and 65 °C for 1 min; then 65 °C for 5 min and 4 °C forever 28 cycles of 94 °C for 30 s; 60 °C for 30 s; and 65 °C for 1 min; then 65 °C for 5 min and 4 °C forever |
Nested forward MDRFR2F25_ CAGATGATGAAATGTTTAAAGATC Nested reverse MDRFR2R25_-TAAATAACATGGGTTCTTGACT | 864 |