Fig. 2

a Representative samples of fluorescent bacteria-fed mosquitoes. Adult female An. darlingi fed fluorescent bacteria Serratia-Adu40 (S) or Pantoea-Ovo3 (P), and control females not fed fluorescent bacteria (C). White arrows indicate fluorescent abdomen. b Fluorescent bacteria extracted from Wild females (WA) previously fed Serratia-Adu40 (S) or Pantoea-Ovo3 (P), and their progeny; eggs (E), Larvae 1st–4th instars (L1-L4), Pupae (P), and adult female F1 (A F1), in NA plates containing ampicillin. All experiments were conducted in triplicate with 10 eggs, 10 larvae, 10 pupae and 10 adult mosquitoes examined in each replicate. Each larvae, pupae, adult or eggs pool sample was homogenized in 1 ml sterile water and centrifuged for 3 min at 8,000 rpm. The pellet was resuspended in 200 µl of sterile water by vortexing and centrifuged again for 1 min at 800 rpm. From the supernatant, 50 µl were plated in each Petri dish. Fluorescent bacteria were detected in 100% of experimental samples analyzed and were never detected in control plates