Inter-study and time-dependent variability of metabolite abundance in cultured red blood cells

Malaria Journal

Table 2 Metabolites that vary substantially across all datasets at all time points relative to 0 h

Major pathway	Subordinate pathway	Metabolites	Mean (SD)
Amino acids	Glutamate metabolism	Glutamate	1.74 (0.48)
	Glutamate metabolism	Glutamate, γ-methyl ester	1.54 (0.33)
	Glutathione metabolism	Ophthalmate	1.96 (0.58)
	Glutathione metabolism	5-Oxoproline	1.92 (0.54)
Carbohydrates	Fructose, mannose, and galactose metabolism	Mannitol/sorbitol	1.99 (0.59)
	Fructose, mannose, and galactose metabolism	Fructose	1.69 (0.54)
	Glycolysis, gluconeogenesis, and pyruvate metabolism	Lactate	2.41 (1.26)
		3-Phosphoglycerate	1.76 (0.40)
		Phosphoenolpyruvate	1.71 (0.27)
	Pentose phosphate pathway	Sedoheptulose-7-phosphate	2.93 (1.16)
Cofactors and vitamins	Haemoglobin and porphyrin metabolism	Heme	1.83 (0.41)
Cofactors and vitamins	Vitamin B6 metabolism	Pyridoxal	2.41 (0.74)
Energy	Tricarboxylic acid cycle	α-Ketoglutarate	2.19 (0.87)
Lipid	Endocannabinoid	Oleoyl ethanolamide	2.22 (1.09)
	Endocannabinoid	Stearoyl ethanolamide	1.75 (0.44)
	Long-chain fatty acid	Erucate (22:1n9)	1.72 (0.44)
Peptide	Gamma-glutamyl amino acid	γ-Glutamylisoleucine^‡	2.33 (0.72)
		γ-Glutamylleucine	2.14 (0.59)
		γ-Glutamylthreonine	2.10 (0.73)

Metabolites that have \(\stackrel{\mathrm{-}}{\text{m}}\) greater than 1.5. Here, \(\stackrel{\mathrm{-}}{\text{m}}\) represents average value of \(\widehat{\text{m}}\) (shown in Fig. 1D). The average was taken across all datasets and time points (except 0 h). ^‡Metabolite identified based on m/z ratio alone with no external standard for validation

ISSN: 1475-2875