Fig. 1

Biochemical characterization of Plasmodium falciparum HRP2 from HB3 culture supernatant, infected erythrocytes, and parasite lysates. A Coomassie-stained SDS-PAGE gel of the purified HRP2 from HB3 culture. A molecular weight ladder is shown in lane 1. The samples in each lane are as follows: 2—unprocessed supernatant, 3—column flow-through, 4—wash one, 5—wash five, 6—wash nine, 7—elute one, 8—elute two, 9—elute three, and 10—elute four. B Western blot of the purified HRP2 protein from the supernatant of HB3 culture. A molecular weight ladder is shown in lane 1. The samples in each lane are as follows: 2—unprocessed supernatant, 3—column flow-through, 4—wash one, 5—wash six, 6—wash seven, 7—wash nine, 8—elute one, and 9—elute four