Fig. 3From: Genetic variations in human ATP2B4 gene alter Plasmodium falciparum in vitro growth in RBCs from Gambian adultsRole of variant ATP2B4 genotype group on PMCA4b expression, function and P. falciparum erythrocytic stage growth. Individuals from homozygous (T/T) genotype (red), heterozygous (T/C) genotype (blue) and wild type (C/C) genotype (green) for the ATP2B4 variant haplotype A Expression of PMCA4b protein in RBCs from homozygous (T/T) donors (n = 29) compared to heterozygous (T/C) donors (n = 29) and wild type (C/C) donors (n = 30). ANOVA followed by Dunn’s multiple comparison testing was used (**** = p > 0.001). B Comparison of calcium expulsion from RBCs indicated by calcium half-life in RBCs from homozygous (T/T), (n = 13), heterozygous (T/C) donors (n = 13) and wild type (C/C) donors (n = 13). ANOVA followed by Dunn’s multiple comparison testing was used (**** = p > 0.001). C Inhibition of parasite growth by PMCA4b inhibitor aurintricarboxylic acid (ATA) in RBCs (n = 3 from wild type (C/C) donors). Inhibitory concentration at 50% (IC50) was calculated using a non-linear regression dose–response model. D Growth of P. falciparum strain FCR3-FMG in vitro in RBCs from homozygous (T/T) donors (n = 27), heterozygous (T/C) donors (n = 35) and wild type (C/C) (n = 30) E Growth of P. falciparum field isolates in vitro in RBCs from homozygous (T/T) donors (n = 3), heterozygous (T/C) donors (n = 3) and wild type (C/C) donors (n = 3) were infected with P. falciparum clinical strain 998. F Growth of P. falciparum field isolates in vitro in RBCs from homozygous (T/T) donors (n = 5), heterozygous (T/C) donors (n = 9) and wild type (C/C) donors (n = 5) were infected with P. falciparum clinical strain 952. Values for all panels (D) (E) and (F) are presented relative to growth in RBCs from wild type (C/C) donors ANOVA (*p < 0.5). Each dot represents the mean result of triplicate growth assays from each donor and the error bars represent SEM. G P. falciparum strain FCR3-FMG invasion in RBCs from homozygous (T/T) donors (n = 12), heterozygous (T/C) donors (n = 15) and wild type (C/C) donors (n = 13), Means were compared using one-way ANOVA (ns)Back to article page