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Table 2 Definition of mosquito behavioural modes (adapted from [17])

From: Impacts of dual active-ingredient bed nets on the behavioural responses of pyrethroid resistant Anopheles gambiae determined by room-scale infrared video tracking

Behavioural mode

Definition

Swooping

Flight tracks without net contact

Visiting

Tracks where extended periods of flight were interspersed with infrequent contacts with the bed net. Contacts were characterized as sharp 80° turns or more in the trajectory, and when multiple contacts occurred with the net, the minimum interval between each contact was 0.4 s (i.e., an interval of at least 20-frames, at 50 frames per second)

Bouncing

Tracks where the mosquito made multiple contacts at intervals of less than 0.4 s with the bed net surface; including tracks with short flights between the contacts, or tracks maintaining contact with the bed net surface without being static. This includes ‘walking’ or ‘probing’ the net with gaps in movement lasting less than 0.75 s

Resting

Tracks where the mosquitoes were static for at least 0.75 s on the net surface, or where the velocity of mosquito movement was less than 1.33 mm/s. Mosquitoes were classified as dead and excluded by limiting resting periods to a maximum of 300 s. No dead mosquitoes were found on nets at the end of any test