Fig. 1

Evaluation for error of assay (EoA) in GIA with anti-RH5 antibodies. P. falciparum 3D7 parasites were cultured using RBCs from four different donors (A, B, C and D). Parasites, which were cultured in each donor’s RBC, were utilized to perform GIA on days 12, 14 and 22. Two human polyclonal antibodies, pAb (a and b) at 10.0 (Hi) or 2.5 (Lo) mg/mL, two rabbit pAbs at 5.0 and 1.25 mg/mL (c), or 10 and 2.5 mg/mL (d), and three human monoclonal antibodies, mAb (e, f and g) at 0.5 and 0.03 mg/mL were tested in each assay. h A linear model fit was performed using data shown in a–g. In the analysis, each sample at each test concentration (Sample effect), RBC donor (A, B, C or D; Donor effect) and assay day (12, 14 or 22; Day effect) were treated as independent variables. “signal” is the variance explained by the Sample effect, “explained” is a sum of variance explained by Donor and Day effects, and “unexplained” is the residual variance of the model. i within the “explained” variance, contributions of Donor and Day effects are shown with the 95% confidence interval (95%CI, error bars)