Table 3 The prominent synchronous correlation cross-peaks for P. falciparum infected RBCs and their assignments, 514.5 nm laser line [8,9,10, 15, 22, 27, 34,35,36,37, 41]
From: Course of Plasmodium infection studied using 2D-COS on human erythrocytes
Synchronous | Asynchronous | ||||
|---|---|---|---|---|---|
Auto-peaks | Assignment | Assignment | Cross-peaks | Assignment | |
2930 | νsCH3; proteins and lipids; PC, outer monolayer | νsCH3; proteins and lipids; PC, outer monolayer | −(2929,3173) | 2 × ν37 (oxyHb) | |
1641 | ν10; Hz; amide I (conf-α) | ν10; Hz; amide I (conf-α); | −(1640,2889) | νasCH2; PC, outer monolayer | |
1585 | ν37; haem; His, νC4 = C5 (Nπ-H), δNH; Asp, νa(COO−) | His, νC4 = C5 (Nπ-H), (Nτ-H); Arg, νs(CN3H5+); Lys, δas(NH3+) | −(1633,3183) | 2 × ν37 (oxyHb) | |
1360 | ν4; haem (deoxy); Trp, νN1 = C8 (νW7); Asp, γ(CH2) | ν37; haem (deoxy; His, νC4 = C5 (Nπ-H) | +(1581,2946) | νs(CH3), PE, internal monolayer | |
ν4; haem (deoxy); | +(1356,2943) | νs(CH3), PE, internal monolayer | |||
Assignment | Cross-peaks | Assignment | ν10; Hz; amide I (conf-α); | +(1642,1651) | amide I (disordered) |
νasCH2; PC, outer monolayer | + (2885,2939) | νs(CH3), PE, internal monolayer | ν19; haem; Tyr, ν(CC) | −(1609,1642) | ν10; Hz; amide I (conf-α); |
ν37; haem (oxy); His, νC4 = C5 (Nπ-H), δNH; | + (1584,3175) | 2 × ν37 (oxyHb) | ν2; Hz; , Asp, νas(COO-); His, νC4 = C5 (Nτ-H) | −(1573,1642) | ν10; Hz; amide I (conf-α); |
His, νC4 = C5 (Nτ-H) | + (1577,2945) | νs(CH3), PE, internal monolayer | Lys, δs(NH3+); His, νC4 = C5 (Nπ-, Nτ-); | −(1530,1642) | ν10; Hz; amide I (conf-α); |
ν4; haem (deoxy); Trp νN1 = C8 (W7) | + (1359,1585) | ν37; haem (oxy); His, νC4 = C5 (Nπ-H), δNH; | Tyr, ν(CC) | −(1519,1642) | ν10; Hz ; amide I (conf-α); |
ν4; haem (deoxy); Trp, νN1 = C8 (W7); Ala | + (1360,1639) | ν10; Hz; | ν4; Hz; Trp, νN1 = C8 (W7) | +(1371,1642) | ν10; Hz; amide I (conf-α); |
ν13 or ν42;haem (oxy); Tyr, ν7a’ ν(CO) | + (1229,1586) | ν37; haem (oxy); His, νC4 = C5 (Nπ-H), δNH; | ν4; haem (deoxy); Trp, νN1 = C8 (W7); Ala | +(1360,1642) | ν10; Hz; amide I (conf-α); |
ν30; haem (deoxy); Asp; Tyr; ν9a,(CH); δ(COH) | + (1171,1588) | ν37; haem (oxy); His, νC4 = C5 (Nπ-H), δNH; | ν4; haem (deoxy); Trp, νN1 = C8 (W7); Ala | −(1359,1573) | ν2; Hz; His, νC4 = C5 (Nτ-H); Asp; νas(COO-) |
ν47; haem (oxy); Phe,(CC) | + (1002,1588) | ν37; haem (oxy); His, νC4 = C5 (Nπ-H), δNH; | ν4; haem (deoxy); Trp, νN1 = C8 (W7); Ala | −(1359,1535) | Amide II; Lys, νCN, δ(NH) |
Thr, γrock(CH2); Ser; Val; | + (746,1589) | ν37; haem (oxy); His, νC4 = C5 (Nπ-H), δNH; | ν4; haem (deoxy); Trp, νN1 = C8 (W7); Ala | +(1359,1374) | ν4; haem (oxy); Asp |
ν4; haem (oxy); Cys,(CS) | + (676,1587) | ν37; haem (oxy); His, νC4 = C5 (Nπ-H), δNH; | ν4; haem (deoxy); | + (671,1642) | ν10; Hz; amide I (conf-α); |
ν7; haem (oxy); Cys,(CS) | + (676,1361) | ν4; haem (deoxy); Trp, νN1 = C8 (W7) | |||