Characteristics of molecular markers associated with chloroquine resistance in Plasmodium vivax strains from vivax malaria cases in Yunnan Province, China

Table 1 The primers names and thieves sequences, target fragment lengths for the nested PCR amplification the pvmdr1 gene in P. vivax strains

Fragment	Nested PCR	Primer names	Primer sequences (5′ → 3′)	Product length (bp)	Amplification interval
F1	First round	DonMD-1-1F	TAACTCCTCACCGTTTGGGAAT	1245	365,143–366,387
	First round	DonMD-1-1R	TCATTGTTTGGTTGCTGGTTGC	1245	365,143–366,387
	Second round	DonMD-1F	GGTGTGTATATCTTGAGTTTGCAT	995	365,277–366,271
	Second round	DonMD-1R	CGTGTACTTACTGTACAGCTTT	995	365,277–366,271
F2	First round	DonMD-2-2F	TTTATTACCATATTTACGTACGCAAG	1385	364,189–365,573
	First round	DonMD-2-2R	ATGATGATCGTAATTCTGTTTTCG	1385	364,189–365,573
	Second round	DonMD-2F	TAACAACACCATGTCCATCATCG	1041	364,360–365,400
	Second round	DonMD-2R	TTAGATGCATTAGAACCCACCAG	1041	364,360–365,400
F3	First round	DonMD-3-3F	CAACATCAAGTATAGTTTGTACAGC	1368	363,295–364,662
	First round	DonMD-3-3R	TGAACATCTCTGTTAATATGTGCTG	1368	363,295–364,662
	Second round	DonMD-3F	TTAGTGTTTCGAAGAAGGTGCA	959	363,489–364,447
	Second round	DonMD-3R	GTAGAGGGAGTACTTATTCGAGT	959	363,489–364,447
F4	First round	DonMD-4-4F	GCAGCATTTATAAGGACTCCG	1387	362,467–363,853
	First round	DonMD-4-4R	CTCATCACGGTAGATTTGCC	1387	362,467–363,853
	Second round	DonMD-4F	GCCATTATAGCCCTGAGCATTAT	1062	362,559–363,620
	Second round	DonMD-4R	GACGTTTGGTCTGGACAAGATATC	1062	362,559–363,620
F5	First round/ Second round	DonMD-5-5F	GAGAAGGCTATTGATTATTCGAAT	1571	361,387–362,957
F5	First round/ Second round	DonMD-5-5R	TTAACTATGTTTACTACGGTTAAGGG	1571	361,387–362,957

ISSN: 1475-2875