Fig. 1

Melting curves of one P. ovale wallikeri (A), one P. vivax (B) and one P. falciparum (parasitaemia < 0.1%) (C) patient followed after starting anti-malarial treatment. The specific melting curve pattern was used to identify the Plasmodium species. The x-axis shows the temperature (°C). The y-axis shows the negative derivative of fluorescence (RFU) with respect to temperature (T). The Plasmodium species is indicated in the left corner of each figure. The amount of days after the start of anti-malarial treatment is indicated at the right of the melting curves. Red curves correspond to the Texas Red labelled probe, purple curves to the Cy5 labelled probe, and brown curves to the Cy5.5 labelled probe. The MC004 assay was reported as negative if no melting curves were present. For the sake of clarity, not all follow-up samples are included in the figures