Performance of a novel melting curve-based qPCR assay for malaria parasites in routine clinical practice in non-endemic setting

Table 1 Agreement between microscopy, RDT and the MC004 assay for patients with suspected malaria

Microscopy		Melting curve-based qPCR (MC004)					RDT	RDT
Microscopy	Pf	Pv	Pm	Pow	Poc	Negative	( +)	(−)
Pf	26	0	0	0	0	0	26	0
Pv	0	2	0	0	0	0	0	3
Pm	0	0	2	0	0	0	0	2
Po	0	0	0	1	1	0	0	2
Mixed	1*	0	0	0	0	0	1**	0
No malaria parasites seen	0	1	0	0	0	270	0	270
Total	27	3	2	1	1	270	27	277

Note the two discrepancies in bold
Pf/Pv/Pm/Pow/Poc/Po = P. falciparum, P. vivax, P. malariae, P. ovale wallikeri, P. ovale curtisi, P. ovale
*Misidentified mixed infection of P. falciparum + P. vivax
**Presence P. falciparum, P. vivax and PAN band

ISSN: 1475-2875