Table 3 Molecular diversity of the Pfk13 propeller gene in Kenya and Ethiopia, 2018-2022
Tajima’s test of neutrality† | Fu and Li’s test of neutrality | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Country | Study site§ | Sample size | Proportion of polymorphic sites | Number of haplotypes, h | Haplotype diversity, Hd ± SD | Nucleotide diversity, πi ± SD (10–3)¶ | Tajima's D | P-value | Fu and Li’s F test statistic | P-value |
Kenya | Homa Bay | 466 | 7/2181 | 8 | 0.055 ± 0.015 | 0.03 ± 0.01 | −1.823 | < 0.05 | −2.345 | < 0.05 |
Kakamega | 126 | 7/2181 | 8 | 0.302 ± 0.053 | 0.15 ± 0.03 | −1.698 | NS | −1.882 | NS | |
Kisii | 12 | 0/2181 | 1 | 0 | 0 | – | – | – | – | |
Kombewa | 179 | 6/2181 | 7 | 0.088 ± 0.029 | 0.04 ± 0.01 | −1.885 | < 0.05 | −3.133 | < 0.02 | |
Subtotal | 738 | 14/2181 | 15 | 0.104 ± 0.015 | 0.05 ± n.d | −2.084 | < 0.01 | −3.771 | < 0.02 | |
Ethiopia | Arjo | 91 | 1/2181 | 2 | 0.022 ± 0.021 | 0.01 ± 0.01 | −1.038 | NS | −1.999 | NS |
Awash | 23 | 0/2181 | 1 | 0 | 0 | – | – | – | – | |
Dire Dawa | 50 | 1/2181 | 2 | 0.040 ± 0.038 | 0.02 ± 0.02 | −1.103 | NS | −1.884 | NS | |
Gambella | 133 | 0/2181 | 1 | 0 | 0 | – | – | – | – | |
Semera | 22 | 1/2181 | 2 | 0.247 ± 0.108 | 0.11 ± 0.05 | −0.175 | NS | 0.479 | NS | |
Subtotal | 319 | 2/2181 | 3 | 0.031 ± 0.014 | 0.01 ± 0.01 | −1.150 | NS | −1.499 | NS | |
Total | 1102 | 15/2181 | 16 | 0.083 ± 0.012 | 0.04 ± n.d | −2.088 | < 0.01 | −3.729 | < 0.02 | |