Table 4 Mutations identified in kelch13 propeller gene of Plasmodium falciparum in abnormal haemoglobin (sickle cell disease) patients
Type of mutation | clinical isolates | Type of Haemoglobin | Sensitivity to DHA | Nucleic acid | Amino-Acid | HbAA | HbAS | HbAC | HbSS | HbSC |
|---|---|---|---|---|---|---|---|---|---|---|
Synonymous | ANK-074 | HbAA | Sensitive | GGC→GGT | G287G | 1 | – | – | – | – |
Non-synonymous | ANK-030 | HbAC | Sensitive | TTA→TTT | L462F | – | – | 1 | – | – |
ANK-035 | HbAA | Sensitive | TGG→GGG | W565G | 1 | – | – | – | – | |
AAT→TAT | N585Y | 1 | – | – | – | – | ||||
GGT→GCT | G595A | 1 | – | – | – | – | ||||
TAT→TCT | Y635S | 1 | – | – | – | – | ||||
GGA→AGG | G450R | 1 | – | – | – | – | ||||
AAT→AAA | N498K | 1 | – | – | – | – | ||||
ANK-054 | HbAA | Sensitive | GTG→GCG | V568A | 1 | – | – | – | – | |
ANK-060 | HbAA | Sensitive | AAT→CAT | N554H | 1 | – | – | – | – | |
TAT→CAC | Y588H | 1 | – | – | – | – | ||||
TTT→GTT | F451V | 1 | – | – | – | – | ||||
GTG→GGT | V520G | 1 | – | – | – | – | ||||
ANK-034 | HbAA | Parasite growth failure | TGT→AGT | C447S | 1 | – | – | – | – | |
TCT→TTT | S549F | 1 | – | – | – | – | ||||
YOP-042 | HbSS | Sensitive | TAT→GAT | Y519D | – | – | – | 1 | ||
Mutations observed | 7 | – | 16 | 16 | 14 | 0 | 1 | 1 | 0 | |
Total sequenced 99 | – | – | – | 57 | 13 | 3 | 17 | 9 | ||