Fig. 2

Prevalence of infection of mosquitoes fed on infected blood with P. falciparum mature stage V gametocytes through the SMFA. Graphic shows the prevalence of infection of mosquitoes fed in three independent experiments. The bars show the mean ± SD. Aseptic = Mosquitoes treated with antibiotics since emergence until 24 h before blood feeding; Septic = Mosquitoes non-treated with antibiotics; Photophase = After blood feeding, mosquitoes were transferred into an incubator in light phase; Scotophase = After blood feeding, mosquitoes were transferred into an incubator in dark phase. Kruskal–Wallis test followed by a Dunn Test for Multiple Comparisons was used to compare the different experimental groups (groups that were statistically significant are represented with an asterisk: * p < 0.1, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Barless conditions were also subjected to analysis but were not significantly different