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Fig. 1 | Malaria Journal

Fig. 1

From: RNAscope in situ hybridization reveals microvascular sequestration of Plasmodium relictum pSGS1 blood stages but absence of exo-erythrocytic dormant stages during latent infection of Serinus canaria

Fig. 1

Study design schematic. Domestic canaries Serinus canaria (n = 14) were inoculated with Plasmodium relictum pSGS1 in March/April 2021. Blood samples were taken, and blood films continuously examined within the first 4 months post infection, and once in December and January, respectively. After parasitaemia was no longer detectable by microscopic examination during winter (January 2022), seven birds from the ‘winter group’ (one died naturally—indicated by obelisk) were dissected and tissue samples investigated for dormant stages. The remaining six birds (‘relapse group’) were continuously blood-checked every 3–4 days during spring (March, April) of the following year to check for re-appearance of parasitaemia. While two birds died before relapse, the other four birds were euthanized and dissected after re-appearance of parasitaemia (relapse or recrudescence) in April 2022. An uninfected control group of six birds was investigated in parallel, six of which were dissected in January 2022 and six in April 2022 (not shown in the schematic)

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Malaria Journal

ISSN: 1475-2875

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