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Fig. 2 | Malaria Journal

Fig. 2

From: RNAscope in situ hybridization reveals microvascular sequestration of Plasmodium relictum pSGS1 blood stages but absence of exo-erythrocytic dormant stages during latent infection of Serinus canaria

Fig. 2

Results of testing the RNAscope in situ hybridization (ISH) assay for detecting Plasmodium relictum in tissue sections. A RNAscope ISH showed bright red labelling of P. relictum stages in a lung section from an infected penguin (positive control) displaying numerous blood and tissue meronts (arrowheads) by HE-staining (insert). B A similar staining pattern was observed in the positive control with chromogenic ISH applying a Plasmodium genus-specific probe. C, D Specificity of the RNAscope assay was confirmed by negative ISH result when applying the RNAscope probe on a sample from an uninfected control bird (C) and by applying a negative control probe (DapB) on a positive control sample (D). Scales = 20 µm

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Malaria Journal

ISSN: 1475-2875

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