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Fig. 3 | Malaria Journal

Fig. 3

From: RNAscope in situ hybridization reveals microvascular sequestration of Plasmodium relictum pSGS1 blood stages but absence of exo-erythrocytic dormant stages during latent infection of Serinus canaria

Fig. 3

Plasmodium relictum pSGS1 blood stages detected during latency (A–E) and after re-appearance of parasitaemia (F–J) in experimentally infected Serinus canaria using RNAscope (A–D, F–I) and chromogenic (E, J) in situ hybridization (ISH). A–E Birds dissected during winter (latency) showing rare blood stage signals (arrows) in capillaries of the lung (A, B, E), liver (C), and brain (D). RNAscope signals were comparable to chromogenic ISH blood stage signals located in infected erythrocytes (E, arrowhead). No exo-erythrocytic stages were found in these birds. F–J Birds dissected after relapse showing plenty blood stage signals (arrows) in capillaries and larger blood vessels of the lung (F, G), heart muscle (H, J) and spleen (I). Scale bars = 50 µm (A, F) and 10 µm (B–E, G–J)

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Malaria Journal

ISSN: 1475-2875

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