RNAscope in situ hybridization reveals microvascular sequestration of Plasmodium relictum pSGS1 blood stages but absence of exo-erythrocytic dormant stages during latent infection of Serinus canaria

Table 1 Parasitaemia intensities, latency, and relapse (re-appearance of parasitaemia) recorded by blood film examinations over the course of experimental Plasmodium relictum pSGS1 infections in domestic canaries Serinus canaria (n = 14)

Bird ID	Group	Infection date	1st Parasitaemia (dpi)	Peak (dpi)	Chronic phase (May-Jul 2021)	1st Winter check (10–12-21)	2nd Winter check (12–01-22)	Dissection date winter group	Relapse (dpi)	Spring parasitaemia (Mar-Apr 2022)	Dissection date relapse group
AH2181	-^a	20-04-21	0.006 (6)	3.0 (13)	0–0.3 (0.047)	N/A^a	N/A^a	13-07-21	–	–	–
AH2203	W	20-04-21	0.004 (6)	0.03 (13)	0–0.02 (0.012)	0.01	0	13-01-22	–	–	–
AH2204	W	23-04-21	0.26 (10)	6.0 (17)	0–0.06 (0.014)	2.2	0	13-01-22	–	–	–
AH2205	W	20-04-21	0.1 (6)	0.4 (13)	0–0.08 (0.033)	0.005	0	13-01-22	–	–	–
AH2206	W	16-04-21	0.03 (10)	0.9 (24)	0–0.1 (0.040)	0.02	0	13-01-22	–	–	–
AH2207	W	16-04-21	0.1 (10)	0.2 (24)	0–0.04 (0.011)	0.01	0	13-01-22	–	–	–
AH2208	W	19-03-21	0.04 (10)	1.0 (18)	0–0.1 (0.016)	0.002	0	13-01-22	–	–	–
AH2209	W	23-04-21	0.24 (10)	2.1 (17)	0–0.06 (0.015)	0.005	0	13-01-22	–	–	–
AH2213	R	20-04-21	0.1 (13)	0.1 (13)	0–0.08 (0.023)	0.1	0	–	N/A^b	N/A^b	04-03-22
AH2214	R	16-04-21	0.1 (10)	0.1 (10)	0–0.05 (0.022)	0.02	0	–	N/A^b	N/A^b	16-03-22
AH2215	R	16-04-21	0.4 (10)	0.4 (10)	0–0.2 (0.051)	0.02	0	–	0.04 (335)	0.03–0.3 (0.081)	27-04-22
AH2216	R	23-04-21	0.67 (10)	0.67 (10)	0–0.01 (0.005)	0.02	0	–	0.007 (332)	0.007–0.06 (0.030)	27-04-22
AH2217	R	19-03-21	0.09 (18)	0.3 (24)	0.003–0.03 (0.009)	0.01	0.005	–	0.007 (367)	0.005–0.03 (0.012)	27-04-22
AH2218	R	16-04-21	0.1 (24)	0.1 (24)	0.02–0.08 (0.038)	0.02	0	–	0.05 (335)	0.02–0.2 (0.094)	27-04-22

W winter group, R relapse group, dpi days post infection, N/A not available
^aBird AH2181 was not assigned to a group because it died early during the experiment (13-07-2021)
^bData on relapse and spring parasitaemia are not available due to death of birds before blood film examinations

ISSN: 1475-2875