Malaria Journal

Table 2 Plasmodium relictum pSGS1 blood stages detected by RNAscope in situ hybridization (ISH) in experimentally infected Serinus canaria examined during winter (blood film-latent infection, n = 7) and after relapse (n = 6)

From: RNAscope in situ hybridization reveals microvascular sequestration of Plasmodium relictum pSGS1 blood stages but absence of exo-erythrocytic dormant stages during latent infection of Serinus canaria

Bird ID	Group	RNAscope ISH results^{a, b, c}
Bird ID	Group	he	lu	li	sp	ki	br	mu	sto	int	tr	oe	thm	thr	ski	bm	ova	tes
AH2203	Winter	+	+	+	+	+	+	+	+	−	+	−	−	N/A	−	−	N/A	N/A
AH2204	Winter	+	+	+	+	+	−	+	+	−	−	−	N/A	−	+	−	N/A	N/A
AH2205	Winter	−	+	+	+	+	−	+	−	−	−	−	−	−	−	−	N/A	N/A
AH2206	Winter	+	+ +	+	+	+	+	+	+	−	−	+	+	−	−	−	N/A	N/A
AH2207	Winter	+	+	−	+	+	−	−	−	−	−	−	−	−	−	−	−	N/A
AH2208	Winter	−	+	−	−	−	−	+	−	−	−	−	N/A	N/A	−	−	N/A	−
AH2209	Winter	−	−	−	−	−	−	−	−	−	−	−	N/A	N/A	−	−	N/A	N/A
AH2213	Relapse	+ +	+ + +	+ +	+ +	+	+	+ + +	+	N/A	+	+	−	N/A	+	−	+	N/A
AH2214	Relapse	+ + +	+ + +	+ + +	N/A	+ + +	+ + +	+ + +	+ +	+ + +	+	+ + +	N/A	N/A	+ + +	+ + +	N/A	+ + +
AH2215	Relapse	+	+ + +	+ + +	+ + +	+	+	+	+	+	N/A	+	N/A	N/A	+	+	N/A	N/A
AH2216	Relapse	+	+	+	+	+	+	+	−	+	+	−	N/A	N/A	−	−	N/A	−
AH2217	Relapse	N/A	+	+	+	+	−	−	−	+	−	−	N/A	N/A	−	−	N/A	−
AH2218	Relapse	+	+ + +	+	+ + +	+ +	+	+	+	+	+	+	+	N/A	+	+	N/A	+

^ahe heart, lu lung, li liver, sp spleen, ki kidney, br brain, mu skeletal muscle, sto stomach, int intestines, tr trachea, oe oesophagus, thm thymus, thr thyroid, ski skin, bm bone marrow, ova ovary, tes testicle
^bRNAscope ISH signals found per 10 fields of view (FOV) at 100 × microscopic magnification: low grade ‘ + ’: 1–50; moderate grade ‘ + + ’: 51–100; high grade ‘ + + + ’: > 100; negative '-': no signals detected
^cN/A not available

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ISSN: 1475-2875

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