Fig. 2
From: Detection of P. malariae using a new rapid isothermal amplification lateral flow assay
Assay workflow and timeline. Sample collection, DNA extraction (and thawing of DNA and reagents, if necessary) times vary by method. Newer methods enable rapid extraction in ≤ 10 min (e.g., QuickExtract DNA, Biosearch Technologies, Hoddesdon, UK), but at higher cost, whereas conventional Chelex extraction can require up to 24 h at lower cost. Incubation temperature can be varied, though 39 °C was optimal for the assay. Visualization of positive results is often possible in < 2 min but reported at 5 min to standardize assay output. Figure made using BioRender