Fig. 1

cNK cell depletion has no effect on the development of experimental MA-ARDS. C57BL/6 mice were infected with P. berghei NK65. Mice received 200 µg of mouse anti-NK1.1 (PK136) antibodies or PBS by i.p. injection at -2, 1, 4 and 7 dpi. Mice were dissected at 9 dpi. Leukocytes were isolated from the lungs according to protocol 1 and flow cytometry was performed. A Schematic representation of the experimental set-up. B Number of pulmonary cNK cells gated as CD45⁺ CD3⁻ DX5⁺. Data from 2 experiments. Each symbol represents data of an individual mouse. n = 8 for uninfected controls (CON), n = 8 for PBS, n = 7 for anti-NK1.1. P-values were indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001. The horizontal black line in each group indicates the median. Statistical differences compared to the CON group are indicated with asterisk above the individual data sets and horizontal lines with asterisk on top indicate significant differences between groups. C Parasitaemia was counted daily starting at 5 dpi using Giemsa-stained blood smears. D Clinical score was monitored daily starting at 5 dpi. C–D Data from four experiments. Data are represented as means ± SEM. n = 19–20 for PBS, n = 16–18 for anti-NK1.1. E Level of alveolar edema was determined based on protein concentration in the BALF. F Unperfused left lung was weighed as a second marker for pulmonary edema. Data from two (F) or four (E) experiments. Each symbol represents data of an individual mouse. n = 12–19 for CON, n = 12–18 for PBS, n = 11–15 for anti-NK1.1. P-values were indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001. The horizontal black line in each group indicates the median. Statistical differences compared to the CON group are indicated with asterisk above the individual data sets and horizontal lines with asterisk on top indicate significant differences between groups