Fig. 2

Appearance of a NK1.1⁺ population upon P. berghei NK65 infection that is not depleted with anti-NK1.1 antibodies. C57BL/6 mice were infected with P. berghei NK65. Mice received 500 µg of anti-NK1.1 (PK136) antibodies or PBS by i.p. injection at 6 dpi. Mice were dissected at 9 dpi. Leukocytes were isolated from the lungs according to protocol 2 and flow cytometry was performed. A Schematic representation of the experimental set-up. B Representative plots showing the appearance of an unknown NK1.1⁺ population (orange gate in upper row) upon P. berghei NK65 infection that is different from the cNK cells (green gate in upper row). Gating on DX5 instead of NK1.1 only shows the cNK cells (green gate in bottom row) (C-D) Number of NK cells present in the lungs gated as (C) CD45⁺ CD3⁻ NK1.1⁺ or as D CD45⁺ CD3⁻ DX5⁺. E Absolute number of unknown population in the lungs. C–E Data from three experiments. Each symbol represents data of an individual mouse. n = 12 for uninfected controls (CON), n = 14 for PBS, n = 11 for anti-NK1.1. P-values were indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001. The horizontal black line in each group indicates the median. Statistical differences compared to the CON group are indicated with asterisk above the individual data sets and horizontal lines with asterisk on top indicate significant differences between groups