Fig. 3

Detailed characterization of unknown population revealed aspecific binding of the anti-NK1.1 antibody. C57BL/6 mice were infected with P. berghei NK65 and dissected at 8 or 9 dpi. Leukocytes were isolated from the lungs according to protocol 2 and flow cytometry was performed. A Representative plots showing the gating of the unknown NK1.1⁺ population (orange gate) and the cNK cell population (green gate) with the appropriate fluorescence minus one (FMO) controls (without anti-CD3 (middle panel) or without anti-NK1.1 staining antibodies (right panel)) of CD45⁺ cells isolated from a P. berghei NK65-infected mouse at 9 dpi. B Absolute cell number of the unknown population in the lungs. Data from two experiments. Each symbol represents data of an individual mouse. n = 10 for uninfected controls (CON), n = 11 for d8, n = 9 for d9. P-values were indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001. The horizontal black line in each group indicates the median. Statistical differences compared to the CON group are indicated with asterisk above the individual data sets and horizontal lines with asterisk on top indicate significant differences between groups. C Representative plots showing the characterization of the unknown population in comparison with the cNK cells based on DX5, CD49a, CD4, CD8, FSC and SSC of a P. berghei NK65-infected mouse at 8 dpi. Representative plots showing staining with the isotype control antibody in comparison to staining with the anti-NK1.1 antibody. D Representative plots showing the expression of NKp46 on the unknown population and cNK cells isolated from a P. berghei NK65-infected mouse at 8 dpi