Fig. 4

Sorting of the unknown population demonstrates a mixture of monocytes and macrophages based on morphology. C57BL/6 mice were infected with P. berghei NK65. Mice were dissected at 8 dpi. Leukocytes were isolated from the lungs according to protocol 2 and fluorescence-activated cell sorting was performed. Cytospins were prepared of sorted cells (T cells, cNK cells, macrophages (macros) and unknown population) to assess morphology. Representative plots demonstrating the gating strategy for the sorting and representative images of the cytospins of the sorted populations are shown