Fig. 5

Aspecific binding of anti-NK1.1 antibodies may be mediated by Fc receptors, such as FcγR4. C57BL/6 mice were infected with P. berghei NK65 and dissected at 8. Leukocytes were isolated from the lungs according to protocol 2 and flow cytometry was performed. (A-B) Representative plots showing the gating of cells based on NK1.1 A or FcγR4 B. Absolute numbers of DX5⁺ CD49a⁻ (C), DX5⁻ CD49a⁺ (D) and DX5⁻ CD49a⁻ (E) cells on CD3⁻ NK1.1⁺ cells (left) or CD3⁻ FcγR4⁺ cells (right). C–E Data from two experiments. Inclusion or exclusion of the outlier in de d8 group does not affect the conclusion from these graphs. Significance was tested between d0 and d8 of CD3⁻ NK1.1⁺ cells and between d0 and d8 of the CD3⁻ FcγR4⁺ cells. Each symbol represents data of an individual mouse. n = 6 for d0 and d8. P-values were indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001. The horizontal black line in each group indicates the median