Fig. 4From: Ageing of Plasmodium falciparum malaria sporozoites alters their motility, infectivity and reduces immune activation in vitroMotility by SMOOT. A PLS-DA of day 14 SPZ (light blue), day 17 SPZ (blue) and day 20 SPZ (dark blue). B Loading plot for PLS-DA. C tSNE of day 14 SPZ (light blue), day 17 SPZ (blue) and day 20 SPZ (dark blue). D Percentage of SPZ from each timepoint per cluster plotted in stacked bars. E Features measured and analysed by SMOOT: frames, velocity, angular dispersion and straightness index. PLS-DA performed with R studio, tSNE with MatlabBack to article page